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Land plants drive photorespiration as higher electron‐sink: comparative study of post‐illumination transient O 2 ‐uptake rates from liverworts to angiosperms through ferns and gymnosperms
Author(s) -
Hanawa Hitomi,
Ishizaki Kimitsune,
Nohira Kana,
Takagi Daisuke,
Shimakawa Ginga,
Sejima Takehiro,
Shaku Keiichiro,
Makino Amane,
Miyake Chikahiro
Publication year - 2017
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12580
Subject(s) - photorespiration , photosynthesis , botany , biology , electron transport chain , sink (geography) , photoinhibition , chlorophyll fluorescence , cyanobacteria , photosystem ii , biophysics , cartography , genetics , bacteria , geography
In higher plants, the electron‐sink capacity of photorespiration contributes to alleviation of photoinhibition by dissipating excess energy under conditions when photosynthesis is limited. We addressed the question at which point in the evolution of photosynthetic organisms photorespiration began to function as electron sink and replaced the flavodiiron proteins which catalyze the reduction of O 2 at photosystem I in cyanobacteria. Algae do not have a higher activity of photorespiration when CO 2 assimilation is limited, and it can therefore not act as an electron sink. Using land plants (liverworts, ferns, gymnosperms, and angiosperms) we compared photorespiration activity and estimated the electron flux driven by photorespiration to evaluate its electron‐sink capacity at CO 2 ‐compensation point. In vivo photorespiration activity was estimated by the simultaneous measurement of O 2 ‐exchange rate and chlorophyll fluorescence yield. All C3 ‐plants leaves showed transient O 2 ‐uptake after actinic light illumination (post‐illumination transient O 2 ‐uptake), which reflects photorespiration activity. Post‐illumination transient O 2 ‐uptake rates increased in the order from liverworts to angiosperms through ferns and gymnosperms. Furthermore, photorespiration‐dependent electron flux in photosynthetic linear electron flow was estimated from post‐illumination transient O 2 ‐uptake rate and compared with the electron flux in photosynthetic linear electron flow in order to evaluate the electron‐sink capacity of photorespiration. The electron‐sink capacity at the CO 2 ‐compensation point also increased in the above order. In gymnosperms photorespiration was determined to be the main electron‐sink. C3–C4 intermediate species of Flaveria plants showed photorespiration activity, which intermediate between that of C3 ‐ and C4 ‐flaveria species. These results indicate that in the first land plants, liverworts, photorespiration started to function as electron sink. According to our hypothesis, the dramatic increase in partial pressure of O 2 in the atmosphere about 0.4 billion years ago made it possible to drive photorespiration with higher activity in liverworts.