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Dehydration rate determines the degree of membrane damage and desiccation tolerance in bryophytes
Author(s) -
Cruz de Carvalho Ricardo,
Catalá Myriam,
Branquinho Cristina,
Marques da Silva Jorge,
Barreno Eva
Publication year - 2017
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12511
Subject(s) - dehydration , desiccation , lipid peroxidation , intracellular , desiccation tolerance , membrane permeability , biophysics , membrane , chemistry , metabolism , biochemistry , cell membrane , respiration , oxidative damage , membrane lipids , oxidative stress , biology , botany
Desiccation tolerant ( DT ) organisms are able to withstand an extended loss of body water and rapidly resume metabolism upon rehydration. This ability, however, is strongly dependent on a slow dehydration rate. Fast dehydration affects membrane integrity leading to intracellular solute leakage upon rehydration and thereby impairs metabolism recovery. We test the hypothesis that the increased cell membrane damage and membrane permeability observed under fast dehydration, compared with slow dehydration, is related to an increase in lipid peroxidation. Our results reject this hypothesis because following rehydration lipid peroxidation remains unaltered, a fact that could be due to the high increase of NO upon rehydration. However, in fast‐dried samples we found a strong signal of red autofluorescence upon rehydration, which correlates with an increase in ROS production and with membrane leakage, particularly the case of phenolics. This could be used as a bioindicator of oxidative stress and membrane damage.