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The effect of polar auxin transport on adventitious branches formation in Gracilaria lichenoides in vitro
Author(s) -
Wang Wenlei,
Li Huanqin,
Lin Xiangzhi,
Zhang Fang,
Fang Baishan,
Wang Zhaokai
Publication year - 2016
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12464
Subject(s) - auxin , explant culture , microbiology and biotechnology , botany , indole 3 acetic acid , in vitro , hydrogen peroxide , biology , chemistry , biochemistry , gene
Seaweed tissue culture ( STC ) is an important micropropagation tool that has been applied for strain improvement, micropropagation and genetic engineering. Because the mechanisms associated with STC are poorly understood, its application to these organisms lags far behind that of tissue culture propagation of higher plants. Auxin, calcium (Ca 2+ ) and hydrogen peroxide ( H 2 O 2 ) fluxes all play key roles during plant growth and development. In this study, we therefore measured indole‐3‐acetic acid, Ca 2+ and H 2 O 2 fluxes of Gracilaria lichenoides explants during adventitious branches ( AB s) formation for the first time using noninvasive micro‐test technology. We confirmed that polar auxin transport ( PAT ) also occurs in the marine red alga G. lichenoides . We additionally found that N‐1‐naphthylphthalamic acid may suppress auxin efflux via ABCB1 transporters and then inhibit AB s formation from the apical region of G. lichenoides segments. The involvement of Ca 2+ and H 2 O 2 fluxes in PAT ‐mediated AB formation in G. lichenoides was also investigated. We propose that complex feedback among Ca 2+ , H 2 O 2 and auxin signaling and response systems may occur during AB s polar formation in G. lichenoides explants, similar to that in higher plants. Our results provide innovative insights that should aid future elucidation of mechanisms operative during STC .