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The carbonic anhydrase domain of plant mitochondrial complex I
Author(s) -
Fromm Steffanie,
Senkler Jennifer,
Zabaleta Eduardo,
Peterhänsel Christoph,
Braun HansPeter
Publication year - 2016
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12424
Subject(s) - carbonic anhydrase , arabidopsis , biology , protein subunit , biochemistry , carbonic anhydrase ii , mitochondrion , domain (mathematical analysis) , mutant , protein domain , function (biology) , microbiology and biotechnology , enzyme , gene , mathematical analysis , mathematics
The mitochondrial NADH dehydrogenase complex (complex I) consists of several functional domains which independently arose during evolution. In higher plants, it contains an additional domain which includes proteins resembling gamma‐type carbonic anhydrases. The Arabidopsis genome codes for five complex I‐integrated gamma‐type carbonic anhydrases ( γCA1 , γCA2 , γCA3 , γCAL1 , γCAL2 ), but only three copies of this group of proteins form an individual extra domain. Biochemical analyses revealed that the domain is composed of one copy of either γCAL1 or γCAL2 plus two copies of the γCA1 / γCA2 proteins. Thus, the carbonic anhydrase domain can have six distinct subunit configurations. Single and double mutants with respect to the γCA / γCAL proteins were employed to genetically dissect the function of the domain. New insights into complex I biology in plants will be reviewed and discussed.