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Leaf aquaporin transcript abundance in peanut genotypes diverging in expression of the limited‐transpiration trait when subjected to differing vapor pressure deficits and aquaporin inhibitors
Author(s) -
Devi M. Jyostna,
Sinclair Thomas R,
Jain Mukesh,
Gallo Maria
Publication year - 2016
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12378
Subject(s) - transpiration , aquaporin , biology , trait , abundance (ecology) , botany , genotype , microbiology and biotechnology , genetics , gene , ecology , photosynthesis , computer science , programming language
A plant trait currently being exploited to decrease crop yield loss under water‐deficit conditions is limited‐transpiration rate ( TR lim ) under high atmospheric vapor pressure deficit ( VPD ) conditions. Although limited genotype comparisons for the TR lim trait have been performed in peanut ( Arachis hypogaea ), no detailed study to describe the basis for this trait in peanut has been reported. Since it has been hypothesized that the TR lim trait may be a result of low leaf hydraulic conductance associated with aquaporins ( AQPs ), the first objective of this study was to examine a possible correlation of TR lim to leaf AQP transcriptional profiles in six peanut cultivars. Five of the studied cultivars were selected because they expressed TR lim while the cultivar York did not. Transcripts of six AQPs were measured. Under exposure to high vapor pressure deficit, cultivar C 76‐16 had decreased AQP transcript abundance for four of the six AQPs but in York only one AQP had decreased abundance. The second objective was to explore the influence of AQP inhibitors mercury and silver on expression of TR lim and AQP transcription profiles. Quantitative RT‐PCR data were compared in cultivars York and C 76‐16, which had the extreme response in TR to VPD . Inhibitor treatment resulted in increased abundance of AQP transcripts in both. The results of these experiments indicate that AQP transcript abundance itself may not be useful in identifying genotypes expressing the TR lim trait under high VPD conditions.