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A homologue of vitamin K epoxide reductase in Solanum lycopersicum is involved in resistance to osmotic stress
Author(s) -
Yu ZhiBo,
Yang XiaoJian,
Du JiaJia,
Wan ChunMei,
Xu JiaNing,
Wang WenJun,
Feng YueGuang,
Wang XiaoYun
Publication year - 2016
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12376
Subject(s) - solanum , vitamin k epoxide reductase , peroxidase , reactive oxygen species , catalase , biochemistry , superoxide dismutase , biology , genetically modified crops , arabidopsis , transgene , proline , chlorophyll , botany , enzyme , gene , cyp2c9 , amino acid , cytochrome p450 , mutant
Vitamin K epoxide reductase ( VKOR ) exists widely in the chloroplasts of higher plants and plays important roles in redox regulation. However, investigations of plant VKOR function have primarily focused on VKOR from Arabidopsis , and knowledge concerning this function is therefore quite limited. In this study, Solanum lycopersicum was used to study the function of VKOR . Transcription level analysis revealed that Sl VKOR ( S. lycopersicum VKOR ) expression was upregulated by salt or drought stress. To further investigate the function of Sl VKOR in plants, we generated sense and antisense transgenic S. lycopersicum homozygotes at T 2 generation plants. Compared with wild‐type ( WT ) plants, the leaf disks of the Sl VKOR overexpression plants retained a much higher percentage of chlorophyll after salt or drought treatment, whereas the antisense transgenic plants displayed an opposite response. The overexpressed plants displayed lower levels of H 2 O 2 and superoxide anion radical (O 2 ·− ) than WT plants, whereas antisense plants accumulated considerably more H 2 O 2 and O 2 ·− . The activities of reactive oxygen scavenger enzymes, including superoxide dismutase, peroxidase, ascorbate peroxidase and catalase, were consistent with the accumulation of reactive oxygen species. Based on these results, we suggest that Sl VKOR is involved in resistance to salt or drought stress.