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Functional diversification of the dehydrin gene family in apple and its contribution to cold acclimation during dormancy
Author(s) -
Falavigna Vítor da Silveira,
Miotto Yohanna Evelyn,
Porto Diogo Denardi,
Anzanello Rafael,
Santos Henrique Pessoa dos,
Fialho Flávio Bello,
MargisPinheiro Márcia,
Pasquali Giancarlo,
Revers Luís Fernando
Publication year - 2015
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12338
Subject(s) - dormancy , biology , meristem , gene , gene family , botany , genetics , gene expression , germination
Dehydrins ( DHN ) are proteins involved in plant adaptive responses to abiotic stresses, mainly dehydration. Several studies in perennial crops have linked bud dormancy progression, a process characterized by the inability to initiate growth from meristems under favorable conditions, with DHN gene expression. However, an in‐depth characterization of DHNs during bud dormancy progression is still missing. An extensive in silico characterization of the apple DHN gene family was performed. Additionally, we used five different experiments that generated samples with different dormancy status, including genotypes with contrasting dormancy traits, to analyze how DHN genes are being regulated during bud dormancy progression in apple by real‐time quantitative polymerase chain reaction ( RT‐qPCR ). Duplication events took place in the diversification of apple DHN family. Additionally, MdDHN genes presented tissue‐ and bud dormant‐specific expression patterns. Our results indicate that MdDHN genes are highly divergent in function, with overlapping levels, and that their expressions are fine‐tuned by the environment during the dormancy process in apple.