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A novel P700 redox kinetics probe for rapid, non‐intrusive and whole‐tissue determination of photosystem II functionality, and the stoichiometry of the two photosystems in vivo
Author(s) -
Jia Husen,
Dwyer Simon A.,
Fan DaYong,
Han Yaqin,
Badger Murray R.,
von Caemmerer Susanne,
Chow Wah Soon
Publication year - 2014
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12235
Subject(s) - p700 , photosystem i , photosystem , absorbance , photosystem ii , chemistry , photosynthetic reaction centre , electron donor , redox , electron transport chain , spinach , photochemistry , photosynthesis , biophysics , analytical chemistry (journal) , electron transfer , biochemistry , biology , chromatography , inorganic chemistry , catalysis
We sought a rapid, non‐intrusive, whole‐tissue measure of the functional photosystem II ( PS II ) content in leaves. Summation of electrons, delivered by a single‐turnover flash to P700 + (oxidized PS I primary donor) in continuous background far‐red light, gave a parameter S in absorbance units after taking into account an experimentally determined basal electron flux that affects P700 redox kinetics. S was linearly correlated with the functional PS II content measured by the O 2 yield per single‐turnover repetitive flash in Arabidopsis thaliana expressing an antisense construct to the PsbO (manganese‐stabilizing protein in PS II) proteins of PS II ( PsbO mutants). The ratio of S to z max (total PS I content in absorbance units) was comparable to the PS II / PS I reaction‐center ratio in wild‐type A. thaliana and in control Spinacea oleracea . Both S and S/z max decreased in photoinhibited spinach leaf discs. The whole‐tissue functional PS II content and the PS II /photosystem I ( PS I) ratio can be non‐intrusively monitored by S and S/z max , respectively, using a quick P700 absorbance protocol compatible with modern P700 instruments.