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Differential expression of a novel gene EaF82a in green and yellow sectors of variegated Epipremnum aureum leaves is related to uneven distribution of auxin
Author(s) -
Hung ChiuYueh,
Umstead Makendra L.,
Chen Jianjun,
Holliday Bronwyn M.,
Kittur Farooqahmed S.,
Henny Richard J.,
Burkey Kent O.,
Xie Jiahua
Publication year - 2014
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12219
Subject(s) - arabidopsis , biology , auxin , gene expression , gene , promoter , protoplast , botany , complementary dna , microbiology and biotechnology , genetics , mutant
EaF82 , a gene identified in previous studies of the variegated plant Epipremnum aureum , exhibited a unique expression pattern with greater transcript abundance in yellow sectors than green sectors of variegated leaves, but lower abundance in regenerated pale yellow plants than in green plants derived from leaf tissue culture. Studies of its full‐length cDNA and promoter region revealed two members with only the EaF82a expressed. Immunoblotting confirmed that EaF82a encodes a 12 kDa protein and its accumulation consistent with its gene expression patterns in different color tissues. Transient expression of EaF82a‐sGFP fusion proteins in protoplasts showed that EaF82a seems to be present in the cytosol as unidentified spots. Sequence motif search reveals a potential auxin responsive element in promoter region. Using transgenic Arabidopsis seedlings carrying EaF82a promoter driving the bacterial uid A ( GUS ) gene, an increased GUS activity was observed when IAA (indole‐3‐acetic acid) concentration was elevated. In E. aureum , EaF82a is more abundant at the site where axillary buds emerge and at the lower side of bending nodes where more IAA accumulates relative to the upper side. The measurement of endogenous IAA levels in different color tissues revealed the same pattern of IAA distribution as that of EaF82a expression, further supporting that EaF82a is an IAA responsive gene. EaF82a expression in etiolated transgenic Arabidopsis seedlings responded to IAA under the influence of light suggesting a microenvironment of uneven light condition affects the EaF82a transcript levels and protein accumulation in variegated leaves.