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A high‐throughput imaging auxanometer for roots and hypocotyls of Arabidopsis using a 2D skeletonizing algorithm
Author(s) -
Fraas Simon,
Niehoff Vera,
Lüthen Hartwig
Publication year - 2014
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12183
Subject(s) - hypocotyl , arabidopsis , auxin , apoplast , throughput , arabidopsis thaliana , mutant , computer science , computational biology , biological system , biology , microbiology and biotechnology , botany , biochemistry , gene , operating system , cell wall , wireless
Next generation phenotyping of auxin response mutants will be greatly facilitated by the ability to record rapid growth responses in roots and hypocotyls at high throughput and at high temporal resolution. As Arabidopsis seedlings are very tiny and fragile, imaging is the only adequate way for data acquisition. As camera‐based systems described before have a limited throughput, we used commercial flatbed scanners to record a large number of simultaneous experiments. We developed Hansa Trace, software for automatically detecting and measuring hypocotyl segments and roots in the images. We validated this system by measuring some well‐characterized growth responses to auxins, non‐auxins, ATPase activators and apoplastic acidification. The method can be shared on a cooperation basis and is able to perform measurements with minimal user intervention.