Premium
Measure for measure: determining, inferring and guessing auxin gradients at the root tip
Author(s) -
Peer Wendy Ann,
Jenness Mark K.,
Murphy Angus S.
Publication year - 2014
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12182
Subject(s) - auxin , indole 3 acetic acid , plant hormone , microbiology and biotechnology , biology , mutant , biochemistry , arabidopsis , biophysics , chemistry , gene
The plant hormone auxin is transported from sites of synthesis to sites of action. Auxin responses are mediated by fast (non‐transcriptional) and slow (transcriptional; ubiquitinylation) responses, which affect physiological changes at cellular and organismal scales. As such, auxin transport vectors regulate programmed and plastic growth responses to optimize growth and development. Here we address some common problems in extrapolating ‘universal’ understanding of auxin transport streams from analyses of loss‐of‐function mutants and auxin transport inhibitors. We also discuss the analytical methods and tools used to directly quantify, measure and infer auxin gradients within the plant [ DR5 : GUS / GFP (beta‐glucuronidase/green fluorescent protein), DII‐VENUS ; surface electrodes, direct quantification]. We discuss the assumptions and limitations of each of these analyses, present comparative summaries of auxin transport methods and assay conditions (diffusion, non‐specific transport and relevant assay conditions), and consider what is actually being transported and measured [labeled‐indole‐3‐acetic acid ( IAA ), IAA metabolites].