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Extracellular matrix‐associated proteome changes during non‐host resistance in citrus– X anthomonas interactions
Author(s) -
Swaroopa Rani Tirupaati,
Podile Appa Rao
Publication year - 2014
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12109
Subject(s) - host (biology) , proteome , extracellular , extracellular matrix , chemistry , biology , microbiology and biotechnology , biochemistry , genetics
Non‐host resistance ( NHR ) is a most durable broad‐spectrum resistance employed by the plants to restrict majority of pathogens. Plant extracellular matrix ( ECM ) is a critical defense barrier. Understanding ECM responses during interaction with non‐host pathogen will provide insights into molecular events of NHR . In this study, the ECM ‐associated proteome was compared during interaction of citrus with pathogen Xanthomonas axonopodis pv. citri ( Xac ) and non‐host pathogen Xanthomonas oryzae pv. oryzae ( Xoo ) at 8, 16, 24 and 48 h post inoculation. Comprehensive analysis of ECM ‐associated proteins was performed by extracting wall‐bound and soluble ECM components using both destructive and non‐destructive procedures. A total of 53 proteins was differentially expressed in citrus– Xanthomonas host and non‐host interaction, out of which 44 were identified by mass spectrometry. The differentially expressed proteins were related to (1) defense‐response (5 pathogenesis‐related proteins, 3 miraculin‐like proteins ( MIR , MIR1 and MIR2 ) and 2 proteases); (2) enzymes of reactive oxygen species ( ROS ) metabolism [Cu/Zn superoxide dismutase ( SOD ), Fe‐ SOD , ascorbate peroxidase and 2‐cysteine‐peroxiredoxin]; (3) signaling (lectin, curculin‐like lectin and concanavalin A‐like lectin kinase); and (4) cell‐wall modification (α‐xylosidase, glucan 1, 3 β‐glucosidase, xyloglucan endotransglucosylase/hydrolase). The decrease in ascorbate peroxidase and cysteine‐peroxiredoxin could be involved in maintenance of ROS levels. Increase in defense, cell‐wall remodeling and signaling proteins in citrus– Xoo interaction suggests an active involvement of ECM in execution of NHR . Partially compromised NHR in citrus against Xoo , upon Brefeldin A pre‐treatment supported the role of non‐classical secretory proteins in this phenomenon.