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Chromium stress response effect on signal transduction and expression of signaling genes in rice
Author(s) -
Trinh NgocNam,
Huang TsaiLien,
Chi WenChang,
Fu ShihFeng,
Chen ChiChien,
Huang HaoJen
Publication year - 2014
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12088
Subject(s) - microbiology and biotechnology , signal transduction , biology , kinase , biochemistry , protein kinase a , transcription factor , gene expression , regulation of gene expression , gene
Hexavalent chromium [Cr( VI )] is a non‐essential metal for normal plants and is toxic to plants at high concentrations. However, signaling pathways and molecular mechanisms of its action on cell function and gene expression remain elusive. In this study, we found that Cr( VI ) induced endogenous reactive oxygen species ( ROS ) generation and Ca 2+ accumulation and activated NADPH oxidase and calcium‐dependent protein kinase. We investigated global transcriptional changes in rice roots by microarray analysis. Gene expression profiling indicated activation of abscisic acid‐, ethylene‐ and jasmonic acid‐mediated signaling and inactivation of gibberellic acid‐related pathways in Cr( VI ) stress‐treated rice roots. Genes encoding signaling components such as the protein kinases domain of unknown function 26, receptor‐like cytoplasmic kinase, LRK10 ‐like kinase type 2 and protein phosphatase 2C , as well as transcription factors WRKY and apetala2/ethylene response factor were predominant during Cr( VI ) stress. Genes involved in vesicle trafficking were subjected to functional characterization. Pretreating rice roots with a vesicle trafficking inhibitor, brefeldin A, effectively reduced Cr( VI )‐induced ROS production. Suppression of the vesicle trafficking gene, Exo70 , by virus‐induced gene silencing strategies revealed that vesicle trafficking is required for mediation of Cr( VI )‐induced ROS production. Taken together, these findings shed light on the molecular mechanisms in signaling pathways and transcriptional regulation in response to Cr stress in plants.