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Genetic variation, heritability, divergence and biomass accumulation of rice genotypes resistant to bacterial blight revealed by quantitative traits and ISSR markers
Author(s) -
Mazid Muhammad S.,
Rafii Mohd Y.,
Hanafi Mohamed M.,
Rahim Harun A.,
Latif Md Abdul
Publication year - 2013
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12054
Subject(s) - biology , upgma , heritability , genetic diversity , relative growth rate , genotype , genetic variation , specific leaf area , botany , veterinary medicine , horticulture , photosynthesis , genetics , growth rate , population , gene , medicine , demography , geometry , mathematics , sociology
A field experiment was carried out in order to evaluate genetic diversity of 41 rice genotypes using physiological traits and molecular markers. All the genotypes unveiled variations for crop growth rate ( CGR ), relative growth rate ( RGR ), net assimilation rate ( NAR ), yield per hill (Yhill −1 ), total dry matter ( TDM ), harvest index ( HI ), photosynthetic rate ( PR ), leaf area index ( LAI ), chlorophyll‐ a and chlorophyll‐ b at maximum tillering stage. The CGR values varied from 0.23 to 0.76 gm cm −2 day −1 . The Yhill −1 ranged from 15.91 to 92.26 g, while TDM value was in the range of 7.49 to 20.45 g hill −1 . PR was found to vary from 9.40 to 22.34 µmol m −2 s −1 . PR expressed positive relation with Yhill −1 . Significant positive relation was found between CGR and TDM (r = 0.61**), NAR and CGR (r = 0.62**) and between TDM and NAR (r = 0.31**). High heritability was found in RGR and Yhill −1 . Cluster analysis based on the traits grouped 41 rice genotypes into seven clusters. A total of 310 polymorphic loci were detected across the 20 inter‐simple sequence repeats ( ISSR ) markers. The UPGMA dendrogram grouped 41 rice genotypes into 11 clusters including several sub‐clusters. The Mantel test revealed positive correlation between quantitative traits and molecular markers (r = 0.41). On the basis of quantitative traits and molecular marker analyses parental genotypes, IRBB54 with MR84 , IRBB60 with MR84 , Purbachi with MR263 , IRBB65 with BR29 , IRBB65 with Pulut Siding and MRQ74 with Purbachi could be hybridized for future breeding program.