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Nitric oxide mediates cold‐ and dehydration‐induced expression of a novel MfHyPRP that confers tolerance to abiotic stress
Author(s) -
Tan Jiali,
Zhuo Chunliu,
Guo Zhenfei
Publication year - 2013
Publication title -
physiologia plantarum
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.351
H-Index - 146
eISSN - 1399-3054
pISSN - 0031-9317
DOI - 10.1111/ppl.12032
Subject(s) - abscisic acid , abiotic stress , proline , dehydration , nitric oxide , biochemistry , osmotic shock , nitrosylation , chemistry , microbiology and biotechnology , biology , amino acid , gene , organic chemistry
Hybrid proline‐rich proteins ( HyPRPs ) are cell wall‐localized proteins, and are frequently responsive to environmental stresses. The coding sequence of a HyPRP cDNA was isolated from Medicago falcata , a forage crop that shows cold and drought tolerance. The predicted MfHyPRP contains a proline‐rich domain at N‐terminus after the signal peptide and a conserved eight‐cysteine motif at the C‐terminus. Higher level of MfHyPRP transcript was observed in leaves than in stems and roots under control conditions, while more MfHyPRP transcript was induced in leaves and stems than in roots after cold treatment. Levels of MfHyPRP transcript and MfHyPRP protein in leaves were induced by cold, dehydration, abscisic acid ( ABA ), hydrogen peroxide ( H 2 O 2 ) and nitric oxide ( NO ), but not responsive to salt stress. The cold‐ or dehydration‐induced expression of MfHyPRP was blocked by scavenger of NO , but not affected by inhibitor of ABA biosynthesis or scavenger of H 2 O 2 . The results indicated that NO , but not ABA and H 2 O 2 , was essential in the cold‐ and dehydration‐induced expression of MfHyPRP . Overexpression of MfHyPRP in tobacco led to increased tolerance to freezing, chilling and osmotic stress as well as methyl viologen‐induced oxidative stress. The increased cold and osmotic stress tolerance was proposed to be associated with improved protection against oxidative damages. It is suggested that NO mediates cold‐ and dehydration‐induced expression of MfHyPRP that confers tolerance to abiotic stress.