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Molecular and physiological characterization of Arabidopsis – Colletotrichum gloeosporioides pathosystem
Author(s) -
Gao Mengze,
Wan Miting,
Yang Liyun,
Zhao Meng,
Liu Xiaojin,
Chen Jiajia,
Liu Pandao,
Jiang Lingyan,
Luo Lijuan
Publication year - 2021
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.13364
Subject(s) - pathosystem , biology , appressorium , colletotrichum , oomycete , plant disease resistance , pathogen , arabidopsis , botany , pectobacterium carotovorum , jasmonic acid , arabidopsis thaliana , conidium , microbiology and biotechnology , gene , genetics , mutant
Abstract Anthracnose is a destructive disease that affects a wide range of crop plants especially in tropical and subtropical regions. Colletotrichum spp. are the major pathogens causing anthracnose. In this study, we collected and identified the pathogen from diseased samples of Stylosanthes , a major tropical forage crop. The ability of the pathogen to naturally infect Arabidopsis thaliana was examined. Sequence analysis of ITS, ACT , CHS , and GAPDH genes showed the pathogen to be Colletotrichum gloeosporioides sensu lato (s.l.), and this was supported further by morphological characterization of representative isolates. The disease symptoms and cellular infection process of aggressive isolates (DZ‐19 and HK‐04) and a weak isolate (CJ‐04) were compared. DZ‐19 and HK‐04 caused more severe disease symptoms on both young seedlings and adult plants of Col‐0 and Ws‐2 ecotypes compared to CJ‐04. Furthermore, the more aggressive isolates showed faster and earlier germination of conidia, formation of appressoria, and growth and development of hyphae during the infection. Genetic analysis of the defence response and expression profiling of defence marker genes demonstrated the involvement of MAP kinase, Ca 2+ ‐dependent protein kinase, salicylic acid, ethylene, and jasmonic acid pathways in the resistance against anthracnose. These results suggest that the Arabidopsis – Colletotrichum gloeosporioides pathosystem should provide a valuable tool for exploring the resistance mechanisms against this pathogen.