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Identification and pathogenicity of Neophysopella species associated with Asian grapevine leaf rust in Brazil
Author(s) -
Santos Ricardo F.,
Primiano Isabela V.,
Amorim Lilian
Publication year - 2021
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.13274
Subject(s) - biology , rust (programming language) , pathogenicity , phylogenetic tree , botany , internal transcribed spacer , crop , horticulture , gene , genetics , agronomy , microbiology and biotechnology , computer science , programming language
Asian grapevine leaf rust (AGLR) causes severe crop losses in Brazilian viticulture, mainly in latitudes <25°S. The purpose of this study was to identify the pathogen(s) involved with AGLR in Brazil, based on phylogenetic and morphological analysis and pathogenicity tests. In total, 56 monouredinial isolates from six Brazilian states were identified using the internal transcribed spacer 2 and the large subunit rRNA gene D1/D2 regions. All 50 isolates from the south‐central region were classified as Neophysopella tropicalis , and the other six isolates from the north‐east region as Neophysopella meliosmae‐myrianthae . This result provides evidence that two pathogen introductions from different sources may have occurred in the country. For both species, paraphyses were cylindrical, incurved, aseptate, and hyaline, while urediniospores were short‐pedicellate, obovoid or obovoid‐ellipsoid, with the wall colourless or pale yellowish, evenly echinulate. Representative isolates from both species caused typical AGLR symptoms on Vitis vinifera 'Merlot' and V . labrusca 'Niagara Rosada'. Overall, regardless of the Neophysopella species, isolates caused similar leaf disease severities. Higher disease severity was observed in Niagara Rosada (average of 40.3% of diseased leaf area) compared to Merlot (20.5%). This study reports, for the first time, the characterization of Neophysopella species associated with AGLR in Brazil.