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Diversity and occurrence of chickpea chlorotic dwarf virus on legumes from Iran
Author(s) -
Shahmohammadi N.,
Dizadji A.,
Bihamta M. R.,
Kvarnheden A.
Publication year - 2020
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.13098
Subject(s) - biology , vicia faba , nicotiana benthamiana , genetic diversity , botany , plant virus , legume , host (biology) , virus , virology , genetics , population , demography , sociology
Chickpea chlorotic dwarf virus (CpCDV; genus Mastrevirus , family Geminiviridae ) is one of the most important legume‐infecting viruses with a wide host range and geographic distribution in Africa and Asia. In Iran, CpCDV is common in chickpea ( Cicer arietinum ), but there is limited information about diversity and infections in plants of other legume species. In the current study, a total of 1671 leaf samples from different pulse crops with symptoms were collected in nine provinces of Iran, and the CpCDV infection status was tested by PCR and/or rolling circle amplification (RCA), resulting in the detection of CpCDV in samples of chickpea, lentil ( Lens culinaris ) and faba bean ( Vicia faba ) from different regions. Sequence analysis of complete genomes of 18 isolates recovered by digestion of RCA products revealed infection with isolates of the strains CpCDV‐A and CpCDV‐F in chickpea, lentil and faba bean. Phylogenetic analysis showed that the Iranian isolates of CpCDV were closely related to previously sequenced isolates of CpCDV‐A and CpCDV‐F. To the authors' knowledge, this is the first report of CpCDV‐F in Iran. Using agroinoculation with infectious clones for one isolate each of CpCDV‐A and CpCDV‐F, infectivity was confirmed in both faba bean and chickpea, with plants developing leaf curling and/or yellowing. Both infectious clones also successfully infected Nicotiana benthamiana resulting in mild yellowing and intensive leaf curling for CpCDV‐A, and dark‐green mosaic, dwarfing and mild leaf curling for CpCDV‐F.