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Systemic necrosis in tomato induced by a Japanese isolate of rehmannia mosaic virus in a temperature‐sensitive manner
Author(s) -
Hamada T.,
Mise K.,
Kiba A.,
Hikichi Y.
Publication year - 2019
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.13006
Subject(s) - tobamovirus , biology , pepper , necrosis , recombinant dna , mottle , plant virus , virology , tobacco mosaic virus , gene , virus , horticulture , genetics
The Japanese isolate of rehmannia mosaic virus (Re MV ‐J) was initially isolated from chilli pepper in Japan. The leaves of diseased chilli pepper plants, which hold no tobamovirus‐resistance genes, have mosaic symptoms. As the symptoms progress, the infected plants develop prominent leaf necrosis and severe leaf fall, followed by stem necrosis and fruit distortion. Additionally, Re MV ‐J systematically infects tomato and this reportedly leads to mottle in tomato when incubated at 20 °C. This study found that Re MV ‐J induces systemic necrosis of tomato – except for cultivar Micro‐Tom – when incubated at 25 °C. To determine the virus factor involved in the induction of temperature‐sensitive systemic necrosis on tomato, intergenic recombinants between Re MV ‐J and tomato mosaic virus were constructed. All recombinant viruses systemically infected tomato similar to Re MV ‐J. Recombinant viruses with the movement protein ( MP ) gene of Re MV ‐J induced systemic necrosis on tomato. Incubation at 20 °C significantly reduced the ability of recombinant viruses with the MP gene of Re MV ‐J to induce systemic necrosis. Thus, the Re MV ‐J MP gene is involved in the temperature‐sensitive induction of systemic necrosis on most tomato cultivars tested. This study is the first to report the involvement of the MP gene in temperature‐sensitive induction of systemic necrosis by tobamovirus on tobamovirus‐susceptible tomato.