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An isolate of Wheat streak mosaic virus from foxtail overcomes Wsm2 resistance in wheat
Author(s) -
Kumssa T. T.,
Rupp J. S.,
Fellers M. C.,
Fellers J. P.,
Zhang G.
Publication year - 2019
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12989
Subject(s) - biology , foxtail , virus , setaria , virology , inoculation , botany , horticulture
Wheat streak mosaic virus ( WSMV ) is an economically important pathogen of wheat ( Triticum aestivum ) causing major yield losses in regions where severe infection occurs. To detect the presence of any new virus or new WSMV isolates, green foxtail ( Setaria viridis ) plants exhibiting virus‐like symptoms were sampled in a summer‐fallowed wheat field at the Agricultural Research Center‐Hays, Kansas State University, Hays, Kansas. These plants were tested serologically for four wheat viruses: WSMV , Triticum mosaic virus (TriMV), High Plains wheat mosaic virus ( HPWM oV) and Foxtail mosaic virus (Fo MV ). Among 38 plant samples exhibiting virus‐like symptoms, 29 contained WSMV as indicated by ELISA . Four isolates from samples with relatively strong reactions were transferred to healthy wheat seedlings by mechanical inoculation in a growth chamber for pathogenicity testing. Three isolates were avirulent to a wheat variety RonL, which contains Wsm2 , a gene providing temperature‐sensitive resistance to currently prevalent isolates of WSMV . However, one isolate, KSH294, was able to infect RonL and showed more virulence on two other varieties/lines containing Wsm2 . Further sequence and phylogenetic analysis of KSH 294 confirmed that this isolate displays a sequence homology with WSMV , but has sequence differences making it distinct from previously identified WSMV isolates included in the phylogenetic analysis.