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Transcriptome analysis of mycorrhizal and nonmycorrhizal soybean plantlets upon infection with Fusarium virguliforme , one causal agent of sudden death syndrome
Author(s) -
Marquez N.,
Giachero M. L.,
Gallou A.,
Debat H. J.,
Declerck S.,
Ducasse D. A.
Publication year - 2019
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12964
Subject(s) - biology , pathosystem , transcriptome , plant defense against herbivory , wrky protein domain , gene , plant disease resistance , thaumatin , microbiology and biotechnology , botany , genetics , gene expression
Soilborne pathogens represent a threat to agriculture causing important yield losses. Sudden death syndrome ( SDS ) of soybean is a severe disease caused by a complex of Fusarium species. This pathosystem has been widely investigated and several strategies have been proposed to manage SDS . Although a decrease in symptoms and in the level of root tissue infection, particularly by F. virguliforme , was observed in the presence of arbuscular mycorrhizal fungi ( AMF ), biological control based on AMF has received less attention. Here, the results are reported of transcriptional analysis of mycorrhizal versus nonmycorrhizal soybean plantlets infected by F. virguliforme , grown under strict in vitro culture experimental conditions. Important transcriptional reprogramming was detected following infection by the pathogen. Results revealed 1768 and 967 differentially expressed genes in the AMF ‐colonized (+ AMF +Fv) and noncolonized (− AMF +Fv) plants, respectively. Major transcriptional changes corresponded to defence response‐related genes belonging to secondary metabolism, stress and signalling categories. The + AMF +Fv treatment showed the largest number of up‐regulated genes related to defence, such as those encoding disease resistance proteins, WRKY transcription factors, auxins, receptors kinases and proteases. Only a few genes had primed expression in the + AMF +Fv treatment, such as those encoding a thaumatin‐like protein ( TLP ) and a pleiotropic drug resistance ( PDR ) protein. Moreover, + AMF +Fv showed a significantly greater number of down‐regulated genes related to cell wall modification and peroxidases than the – AMF +Fv treatment. This detailed investigation increases knowledge of transcriptional changes and potential metabolic pathways involved in the enhanced resistance or tolerance of mycorrhizal plants to infection by F. virguliforme .