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Phylogeny, distribution and pathogenicity of Lasiodiplodia species associated with dieback of table grape in the main Brazilian exporting region
Author(s) -
Correia K. C.,
Silva M. A.,
Morais M. A.,
Armengol J.,
Phillips A. J. L.,
Câmara M. P. S.,
Michereff S. J.
Publication year - 2016
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12388
Subject(s) - lasiodiplodia theobromae , biology , botryosphaeria , table grape , conidium , internal transcribed spacer , botany , horticulture , phylogenetic tree , cultivar , biochemistry , gene
Botryosphaeria dieback is an important disease of table grape in the São Francisco Valley, the main Brazilian exporting region. The objectives of this study were to identify species of Lasiodiplodia associated with botryosphaeria dieback of table grapes in the São Francisco Valley, investigate the prevalence and distribution of the species in the region, and evaluate their pathogenicity and virulence in green shoots of table grape. A total of 112 Lasiodiplodia isolates were obtained from 14 vineyards, located in Casa Nova, Juazeiro and Petrolina. Fungal identifications were made using phylogenetic analysis based on partial sequences of translation elongation factor 1‐ α ( EF 1‐α ) and internal transcribed spacer ( ITS ) sequences, in combination with morphometric characteristics of conidia. Eight species of Lasiodiplodia were identified: L. brasiliense , L. crassispora , L. egyptiacae , L. euphorbicola , L. hormozganensis , L. jatrophicola , L. pseudotheobromae and L. theobromae . Except for L. crassispora , L. pseudotheobromae and L. theobromae , all the other species are reported for the first time on grapevine worldwide. The distribution of Lasiodiplodia species differed between the three table grape populations of São Francisco Valley. All Lasiodiplodia species isolated in this study were present in the population of Casa Nova and Lasiodiplodia theobromae was the most prevalent. All species of Lasiodiplodia were pathogenic on detached green shoots of grapevine, with L. brasiliense being the most virulent.