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Overexpression of barley oxalate oxidase gene induces partial leaf resistance to Sclerotinia sclerotiorum in transgenic oilseed rape
Author(s) -
Liu F.,
Wang M.,
Wen J.,
Yi B.,
Shen J.,
Ma C.,
Tu J.,
Fu T.
Publication year - 2015
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12374
Subject(s) - sclerotinia sclerotiorum , sclerotinia , biology , hydrogen peroxide , genetically modified crops , oxalate , oxidase test , microbiology and biotechnology , botany , transgene , horticulture , biochemistry , gene , chemistry , enzyme , organic chemistry
Sclerotinia stem rot ( SSR ) is a severe disease of oilseed rape, which severely impacts the crop productivity worldwide. Sclerotinia sclerotiorum causes SSR , resulting in the secretion of oxalic acid ( OA ), which can be further degraded to carbon dioxide ( CO 2 ) and hydrogen peroxide (H 2 O 2 ) by oxalate oxidase ( OXO ). In the present investigation, the barley oxalate oxidase ( BOXO , Y14203) gene was introduced into oilseed rape by Agrobacterium ‐mediated transformation to investigate the mechanism by which OXO promotes resistance to S. sclerotiorum . Compared to the control 72 h post‐inoculation, there were c . 15–61% fewer lesions on leaves of the transgenic oilseed rape, which thus exhibited a detectable level of partial resistance in leaf tissue to S. sclerotiorum . Transgenic oilseed rape also showed decreased oxalate and increased hydrogen peroxide levels compared to the control, and the expression of defence response genes involved in the hydrogen peroxide signalling pathway was also induced. Therefore, the improved resistance of oilseed rape could be attributed to the enhanced OA metabolism, production of hydrogen peroxide and the hydrogen peroxide‐mediated defence levels during infection.