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Significant variation in sensitivity to a DMI fungicide in field populations of F usarium graminearum
Author(s) -
Talas F.,
McDonald B. A.
Publication year - 2015
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12280
Subject(s) - propiconazole , biology , fungicide , fusarium , mycotoxin , heritability , pesticide resistance , veterinary medicine , genetics , botany , agronomy , pesticide , medicine
Fusarium head blight caused by Fusarium graminearum (Fg) is a destructive disease on cereals around the world. Intensive application of fungicides on cereals has selected for resistance in many cereal pathogens. Two hundred and twenty‐four isolates of Fg from 13 different German field populations, 22 isolates from the UK and six reference isolates from Italy and the USA were tested for sensitivity to propiconazole. Propiconazole sensitivity showed a high heritability ( H 2 = 0·97), with mean EC 50 values ranging from 5·4 to 62·2 mg L −1 . Nearly 80% of the variance for propiconazole sensitivity was distributed within field populations, suggesting significant potential for selection of resistant isolates at the field scale. Sequence analysis revealed that the CYP 51A and CYP 51C genes carried several non‐synonymous substitutions but the CYP 51B protein was not polymorphic. The non‐synonymous substitutions were distributed randomly among populations and were not correlated with fungicide sensitivity. Although not conclusive, the findings suggest that genes outside of the CYP 51 family may make the most important contribution to DMI resistance in F. graminearum .