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Genetic and phenotypic characterization of Botrytis calthae
Author(s) -
Plesken C.,
Westrich L.D.,
Hahn M.
Publication year - 2015
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12240
Subject(s) - botrytis cinerea , biology , botrytis , conidium , mycelium , botany , fungus , germination , fungicide , host (biology) , pathogenic fungus , pathogen , microbiology and biotechnology , genetics
Botrytis calthae is a necrotrophic plant pathogen, closely related to the ubiquitous broad host range fungus Botrytis cinerea , but highly host specific. Botrytis isolates from lesions of Caltha palustris grown at different locations were classified with genetic markers as either B. calthae or Botrytis pseudocinerea , or less frequently as B. cinerea . A PCR‐based identification of B. calthae was developed. Seven haplotypes of B. calthae could be distinguished. Compared to B. cinerea , mycelium growth of B. calthae was similar, but conidiation less abundant, and sclerotia formation was only partially repressed by light. Conidia of B. calthae germinated more slowly, and showed a highly acidic optimum ( pH 2·5) compared to B. cinerea conidia ( pH 5·3). All B. calthae isolates were sensitive to common anti‐ Botrytis fungicides, but showed partial resistance to the succinate dehydrogenase inhibitors boscalid, fluopyram and carboxin. Infection experiments revealed a weak capability of B. calthae to induce necrotic lesions on plants that are hosts for B. cinerea . On C. palustris leaves, B. calthae induced similar lesions to B. cinerea . These data provide a basis for comparative molecular investigation of the physiology and host specificity of B. calthae and closely related Botrytis species.