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Detection of T omato yellow leaf curl virus in imported tomato fruit in northern E urope
Author(s) -
Just K.,
Leke W. N.,
Sattar M. N.,
Luik A.,
Kvarnheden A.
Publication year - 2014
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12205
Subject(s) - begomovirus , tomato yellow leaf curl virus , biology , horticulture , plant virus , polymerase chain reaction , leaf curl , sequence analysis , nucleic acid sequence , virology , botany , virus , gene , genetics
Imported tomato fruits infected with T omato yellow leaf curl virus ( TYLCV ) were identified on the market in northern E urope using paper‐based FTA C lassic C ards ( W hatman), polymerase chain reaction ( PCR ) and partial DNA sequence analysis. Trade tomatoes originating from southern E urope, A frica and the M iddle E ast were sampled in E stonia and S weden, and tested for infection with begomoviruses. Out of 100 batches analysed with five fruits sampled in each batch (58 batches from Estonia and 42 from S weden), 20 batches were positive (16 from E stonia and four from S weden). Rolling circle amplification ( RCA ) and full‐length genome sequence analysis of one isolate collected in Estonia and one isolate in Sweden, revealed highest nucleotide sequence identity at 99% to TYLCV ‐ IL for the Estonian isolate and at 97% to TYLCV ‐ M ld for the S wedish isolate. In this study, TYLCV was identified for the first time in imported tomato fruits on the market in northern E urope. FTA cards proved to be an effective means to collect, extract and store begomovirus DNA from tomato fruits and the subsequent molecular analysis.