z-logo
Premium
Detection of T omato yellow leaf curl virus in imported tomato fruit in northern E urope
Author(s) -
Just K.,
Leke W. N.,
Sattar M. N.,
Luik A.,
Kvarnheden A.
Publication year - 2014
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12205
Subject(s) - begomovirus , tomato yellow leaf curl virus , biology , horticulture , plant virus , polymerase chain reaction , leaf curl , sequence analysis , nucleic acid sequence , virology , botany , virus , gene , genetics
Imported tomato fruits infected with T omato yellow leaf curl virus ( TYLCV ) were identified on the market in northern E urope using paper‐based FTA C lassic C ards ( W hatman), polymerase chain reaction ( PCR ) and partial DNA sequence analysis. Trade tomatoes originating from southern E urope, A frica and the M iddle E ast were sampled in E stonia and S weden, and tested for infection with begomoviruses. Out of 100 batches analysed with five fruits sampled in each batch (58 batches from Estonia and 42 from S weden), 20 batches were positive (16 from E stonia and four from S weden). Rolling circle amplification ( RCA ) and full‐length genome sequence analysis of one isolate collected in Estonia and one isolate in Sweden, revealed highest nucleotide sequence identity at 99% to TYLCV ‐ IL for the Estonian isolate and at 97% to TYLCV ‐ M ld for the S wedish isolate. In this study, TYLCV was identified for the first time in imported tomato fruits on the market in northern E urope. FTA cards proved to be an effective means to collect, extract and store begomovirus DNA from tomato fruits and the subsequent molecular analysis.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here