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Components of different signalling pathways regulated by a new orthologue of A t PROPEP 1 in tomato following infection by pathogens
Author(s) -
Trivilin A. P.,
Hartke S.,
Moraes M. G.
Publication year - 2014
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12190
Subject(s) - biology , gene silencing , solanum , gene , pathogen , in silico , rna interference , genetics , microbiology and biotechnology , botany , rna
Plants express different defence mechanisms in response to pathogens. Understanding the recognition of pathogen‐associated molecular patterns ( PAMP s) by specific receptors, and the role of endogenous signals such as A t P ep1 that regulate expression of genes in A rabidopsis thaliana , has aided the understanding of the defence mechanisms in different species. The aim of this study was to identify possible orthologous sequences of At PROPEP s in tomato ( S olanum lycopersicum ) and characterize its role in resistance to necrotrophic pathogens. The presence of an orthologue of the A . thaliana At PROPEP 1 gene in S . lycopersicum , Sl PROPEP , by in silico analysis, is reported here. This has 96% identity with the C ‐terminal region of a previously described potato peptide, another possible orthologue of At P ep1. A virus‐induced gene silencing ( VIGS ) system was employed to investigate the role of the SlPROPEP . Silencing of SlPROPEP in tomato made plants more susceptible to P ythium dissotocum ; approximately 30% of SlPROPEP ‐silenced plants showed stem constriction compared with 4% in control plants. Furthermore, quantification of P . dissotocum by qPCR revealed that the increase in symptom severity in SlPROPEP ‐silenced plants was associated with a 15 times increase in growth of the pathogen compared to control plants. Silencing of SlPROPEP also resulted in decreased expression of genes involved in plant defence against pathogens, such as PR‐1 , PR ‐5 , ERF 1 , LOX ‐ D and DEF 2 . These results suggest that SlPROPEP is involved in tomato resistance to P . dissotocum and probably acts as a pathogen‐associated molecular pattern through signalling pathways mediated by jasmonic acid/ethylene ( JA/ET ).