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Biological and molecular variation of Cherry leaf roll virus isolates from Malus domestica , Ribes rubrum , Rubus idaeus , Rumex obtusifolius and Vaccinium darrowii
Author(s) -
Woo E. N. Y.,
Pearson M. N.
Publication year - 2014
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12166
Subject(s) - biology , rubus , botany , herbaceous plant , ribes , horticulture
Cherry leaf roll virus ( CLRV ) isolates from M alus domestica , R ibes rubrum , R ubus idaeus , R umex obtusifolius and V accinium darrowii were characterized based on nucleotide sequences of a 371 bp fragment of the 3′ untranslated region ( UTR ) of their genomic RNA s, symptoms in the herbaceous hosts, C henopodium amaranticolor , C henopodium quinoa , N icotiana benthamiana , N icotiana occidentalis and N icotiana tabacum , and seed transmission in N . occidentalis . The different isolates induced a range of localized and systemic disease symptoms, of varying severity, in the herbaceous hosts. The isolates from M . domestica , R . rubrum , R . obtusifolius and V . darrowii all showed greater than 80% seed transmission in N . occidentalis , but no seed transmission was observed for the R . idaeus isolate. Based on symptoms and seed transmission, the isolates appear to be biologically distinct strains of CLRV . Phylogenetic analysis of the nucleotide sequences from the 3′ UTR , commonly used to detect CLRV , showed that four isolates from M . domestica , R . rubrum , R . idaeus and V . darrowii were almost identical but an isolate from R . obtusifolius exhibited a pairwise nucleotide difference of up to 5·4% when compared to these isolates. There was no obvious correlation between sequence differences and symptomatology.