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Construction of a cassava PR protein‐interacting network during X anthomonas axonopodis pv. manihotis infection
Author(s) -
Román V.,
BossaCastro A. M.,
Vásquez A.,
Bernal V.,
Schuster M.,
Bernal N.,
López C.
Publication year - 2014
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12155
Subject(s) - chitinase , biology , gene , yeast , gene ontology , function (biology) , genetics , two hybrid screening , microbiology and biotechnology , computational biology , gene expression
Pathogenesis‐related ( PR ) proteins are inducible antimicrobial defence proteins whose specific function in immunity or in other plant processes have not been deeply studied. Genes coding for PR s have been reported in different plant species with numerous homologous proteins belonging to the same functional protein family. Previously some PR candidate genes were identified in response to cassava bacterial blight ( CBB ) caused by X anthomonas axonopodis pv. manihotis ( X am): the genes for hevamine ( HEV ), chitinase ( CHI ) and sulphite reductase ( S i R ) were induced in a resistant cassava cultivar during the response to X am. To understand their functional roles in defence responses, a protein–protein interaction map was generated based on yeast two‐hybrid ( Y 2 H ) assays of these candidate PR proteins. The results showed that the cassava PR s interact with each other and, although hevamine and chitinase belong to the same class of chitinases, they share only four interactors. Co‐regulated expression of PR s and their interactors was observed and similar ontology terms were identified for interactors. Furthermore, an overlap between immune and other metabolic pathways was noticed based on other bioinformatics studies. These results therefore shed more light on the possible function of PR proteins.