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Expression of signalling and defence‐related genes mediated by over‐expression of JERF 1 , and increased resistance to sheath blight in rice
Author(s) -
Pan X.,
Li Y.,
Zhang H.,
Huang R.,
Liu W.,
Ming J.,
Liu S.,
Li X.
Publication year - 2014
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12064
Subject(s) - abscisic acid , biology , gene , methyl jasmonate , phenylalanine ammonia lyase , gene expression , promoter , genetically modified rice , transcription factor , plant disease resistance , lipoxygenase , microbiology and biotechnology , oryza sativa , genetics , transgene , genetically modified crops , enzyme , biochemistry , phenylalanine , amino acid
Sheath blight ( SB ) is one of the most destructive rice diseases that affect the yield and quality of rice. J asmonate and E thylene R esponse F actor 1 , designated JERF 1 , can be induced by ethylene, methyl jasmonate ( M e JA ), abscisic acid (ABA) and salt stress. Previous research demonstrated that over‐expression of JERF 1 enhanced drought tolerance by regulating the expression of stress‐related genes in rice. The present study reports that JERF 1 could improve SB resistance of transgenic rice. At the molecular and physiological levels, JERF 1 could up‐regulate the expression of signalling and defence‐related genes and increase the activities of lipoxygenase ( LOX ) and phenylalanine ammonia lyase ( PAL ). Analysis of cis ‐elements showed that there were several DRE / CRT (dehydration‐responsive element/ C ‐repeat) and/or GCC boxes in the promoters of these candidate genes, indicating that JERF 1 might regulate the expression of defence‐related genes via interaction with these cis ‐elements. These results suggest that JERF 1 might improve SB resistance by modulating the expression of defence‐related genes. This may prove to be a useful strategy for genetic engineering to improve SB resistance using transcription factor genes in rice.