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Characterization and host range of five tumorigenic A grobacterium tumefaciens strains and possible application in plant transient transformation assays
Author(s) -
Hwang H.H.,
Wu E. T.,
Liu S.Y.,
Chang S.C.,
Tzeng K.C.,
Kado C. I.
Publication year - 2013
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12046
Subject(s) - agrobacterium tumefaciens , transfer dna , biology , ti plasmid , transformation (genetics) , plasmid , octopine , gall , bacteria , wild type , genetics , dna , gene , microbiology and biotechnology , mutant , botany
A grobacterium tumefaciens is a G ram‐negative bacterium. It causes plants to produce crown gall disease because of the transfer, integration and expression of oncogenes encoded by the T ‐ DNA (transferred DNA ) region of the tumour‐inducing ( T i) plasmid. A set of transferred genes directs the production of bacterial nutrients, called opines, formed by condensation of an amino acid and a keto acid or a sugar. Transformed cells synthesize and secrete substantial quantities of particular opines, which A . tumefaciens then uses as a carbon and sometimes nitrogen source. A grobacterium tumefaciens strains are usually classified on the basis of the opines they can catabolize. Because of the ability to transfer DNA between different kingdoms, A . tumefaciens is also frequently used to generate transgenic plants. This study analyses five poorly characterized wildtype A grobacterium strains, 1D1108, 1 D 1460, 1 D 132, 1 D 1478 and 1 D 1487, isolated from E uonymus , cane, cherry, S alix and apple, respectively. Partial T i‐plasmid sequence analysis demonstrated that the five strains harbour the nopaline‐type T i plasmid. Tumorigenesis and transient transformation assays of the five analysed and six wildtype A . tumefaciens strains were performed with selected plant species, including two or three species of B rassicaceae, A steraceae, S olanaceae, A piaceae and L eguminosae. The A . tumefaciens strains 1 D 1108, 1 D 1460 and 1 D 1478 showed higher transformation efficiencies than the previously characterized A . tumefaciens strains with several economically important crops. These data suggest the potential use of these newly characterized wildtype A . tumefaciens strains in transient transformation assays with certain plant species.