Premium
Occurrence of Potato virus Y strain PVY NTN in foundation seed potatoes in J apan, and screening for symptoms in J apanese potato cultivars
Author(s) -
Chikh Ali M.,
Karasev A. V.,
Furutani N.,
Taniguchi M.,
Kano Y.,
Sato M.,
Natsuaki T.,
Maoka T.
Publication year - 2013
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12031
Subject(s) - potato virus y , cultivar , biology , potyviridae , potyvirus , strain (injury) , recombinant dna , virus , gene , virology , plant disease resistance , potato virus x , horticulture , plant virus , botany , genetics , anatomy
In 2008 and 2009 seasons, a sudden increase in P otato virus Y ( PVY ) incidence was recorded in foundation seed potatoes in H okkaido, northern J apan. This increase was obvious during the field inspection and the postharvest indexing. Molecular typing revealed that besides the previously reported strains of PVY O and PVY NA‐N , the most common strain identified was the recombinant PVY NTN , with three characteristic recombinant junctions at the HC ‐ P ro, VP g and CP regions. No potato tuber necrotic ringspot disease ( PTNRD ) was observed in foundation seed potatoes in correlation with the presence of PVY NTN . Moreover, an isolate with a typical PVY NTN recombinant genome, namely E u‐12 J p, did not induce PTNRD in 62 Japanese potato cultivars tested in both primarily and secondarily infected plants. Two cultivars carrying the extreme resistance gene R y chc were resistant to the infection with E u‐12 J p, which presents potential sources of resistance to PVY NTN . Eu‐12 J p induced systemic mottle in potato cultivars D esiree and K ing E dward carrying resistance genes N y and N c , respectively, but induced a hypersensitive reaction in potato cultivar M aris B ard, with the N z hypothetical resistance gene typical of the PVY Z strain group. Therefore, based on the genome structure and the reaction of the potato N resistance genes, E u‐12 J p should be classified as PVY Z ‐ NTN , as described for isolates from Idaho, USA recently. This is the first report of PVY Z ‐ NTN in Japan and the sudden and increased occurrence of PVY NTN / PVY Z ‐ NTN represents a potential risk of PTNRD developing and increases the significance of PVY in Japan.