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Fast real‐time detection of Potato spindle tuber viroid by RT‐LAMP
Author(s) -
Lenarčič R.,
Morisset D.,
Mehle N.,
Ravnikar M.
Publication year - 2013
Publication title -
plant pathology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.928
H-Index - 85
eISSN - 1365-3059
pISSN - 0032-0862
DOI - 10.1111/ppa.12017
Subject(s) - potato spindle tuber viroid , consumables , reverse transcription loop mediated isothermal amplification , viroid , biology , reverse transcription polymerase chain reaction , real time polymerase chain reaction , virology , loop mediated isothermal amplification , reverse transcriptase , horticulture , polymerase chain reaction , plant virus , genetics , messenger rna , gene , chemistry , dna , virus
This paper reports the development of a single tube, real‐time, reverse transcription loop‐mediated isothermal amplification (RT‐LAMP) assay for detecting Potato spindle tuber viroid (PSTVd), one of the quarantine pathogens of potato in Europe and North America. The method enables detection of a broad range of PSTVd isolates, and is about 10 times more sensitive than the conventional reverse transcription polymerase chain reaction (RT‐PCR) assay. Its benefits are not only its speed (15–25 min to obtain results) and cost effectiveness (resulting from time saved as well as cheaper consumables), but also its demonstrated ability to be performed in the field, using portable instruments.