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Influence of salt stress on C 4 photosynthesis in Miscanthus sinensis Anderss.
Author(s) -
Sun Q.,
Yamada T.,
Han Y.,
Takano T.
Publication year - 2021
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.13192
Subject(s) - photosynthesis , phosphoenolpyruvate carboxylase , salinity , biology , photosynthetic capacity , chlorophyll fluorescence , chlorophyll , botany , transpiration , rubisco , photosynthetic efficiency , miscanthus sinensis , horticulture , miscanthus , bioenergy , ecology , biofuel
Miscanthus sinensis Anderss. is a good candidate for C 4 bioenergy crop development for marginal lands. As one of the characteristics of marginal lands, salinization is a major limitation to agricultural production. The present work aimed to investigate the possible factors involved in the tolerance of M. sinensis C 4 photosynthesis to salinity stress. Seedlings of two accessions (salt‐tolerant ‘JM0119’ and salt‐sensitive ‘JM0099’) were subjected to 0 m m NaCl (control) or 250 m m NaCl (salt stress treatment) for 2 weeks. The chlorophyll content, parameters of photosynthesis and chlorophyll a fluorescence, activity of C 4 enzymes and expression of C 4 genes were measured. The results showed that photosynthesis rate, transpiration rate, chlorophyll content, PSII operating efficiency, coefficient of photochemical quenching, activity of phospho enol pyruvate carboxylase (PEPC) and pyruvate, orthophosphate dikinase (PPDK) and gene expression of PEPC and PPDK under salinity were higher after long‐term salinity exposure in ‘JM0119’ than in ‘JM0099’, while activity of NADP‐malate dehydrogenase (NADP‐MDH) and NADP‐malic enzyme (NADP‐ME), together with expression of NADP‐MDH and NADP‐ME , were much higher in ‘JM0099’ than in ‘JM0119’. In conclusion, the increased photosynthetic capacity under long‐term salt stress in the salt‐tolerant relative to the salt‐sensitive M. sinensis accession was mainly associated with non‐stomatal factors, such as reduced chlorophyll loss, higher PSII operating efficiency, enhanced activity of PEPC and PPDK and relatively lower activity of NADP‐ME.