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Peanut ( Arachis hypogaea L.) S‐adenosylmethionine decarboxylase confers transgenic tobacco with elevated tolerance to salt stress
Author(s) -
Meng D.Y.,
Yang S.,
Xing J.Y.,
Ma N.N.,
Wang B.Z.,
Qiu F.T.,
Guo F.,
Meng J.,
Zhang J.l.,
Wan S.B.,
Li X.G.
Publication year - 2021
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.13173
Subject(s) - spermidine , biology , polyamine oxidase , arachis hypogaea , polyamine , arachis , proline , spermine , biochemistry , carboxy lyases , botany , enzyme , horticulture , amino acid
Polyamines play an important role in stress response. In the pathway of polyamines synthesis, S-adenosylmethionine decarboxylase (SAMDC) is one of the key enzymes. In this study, a full length cDNA of SAMDC ( AhSAMDC ) was isolated from peanut ( Arachis hypogaea L.). Phylogenetic analysis revealed high sequence similarity between AhSAMDC and SAMDC from other plants. In peanut seedlings exposed to sodium chloride (NaCl), the transcript level of AhSAMDC in roots was the highest at 24 h that decreased sharply at 72 and 96 h after 150 mM NaCl treatment. However, the expression of AhSAMDC in peanut leaves was significantly inhibited, and the transcript levels in leaves were not different compared with control These results implied the tissue‐specific and time-specific expression of AhSAMDC . The physiological effects and functional mechanism of AhSAMDC were further evaluated by overexpressing AhSAMDC in tobaccos. The transgenic tobacco lines exhibited higher germination rate and longer root length under salt stress. Reduced membrane damage, higher antioxidant enzyme activity, and higher proline content were also observed in the transgenic tobacco seedlings. What's more, AhSAMDC also led to higher contents of spermidine and spermine, which can help to scavenge reactive oxygen species. Together, this study suggests that AhSAMDC enhances plant resistance to salt stress by improving polyamine content and alleviating membrane damage