Premium
Deep‐sequencing of Solanum commersonii small RNA libraries reveals riboregulators involved in cold stress response
Author(s) -
Esposito S.,
Aversano R.,
Bradeen J. M.,
Di Matteo A.,
Villano C.,
Carputo D.
Publication year - 2020
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12955
Subject(s) - biology , rna , microrna , transcriptome , genetics , deep sequencing , gene , cold stress , non coding rna , genome , phenotype , coding region , small rna , gene expression , computational biology
Among wild species used in potato breeding, Solanum commersonii displays the highest tolerance to low temperatures under both acclimated ( ACC ) and non‐acclimated ( NACC ) conditions. It is also the first wild potato relative with a known whole genome sequence. Recent studies have shown that abiotic stresses induce changes in the expression of many small non‐coding RNA (snc RNA ). We determined the small non‐coding RNA (snc RNA ome) of two clones of S. commersonii contrasting in their cold response phenotypes via sm RNA seq. Differential analysis provided evidence that expression of several mi RNA s changed in response to cold stress conditions. Conserved miR408a and miR408b changed their expression under NACC conditions, whereas miR156 and miR169 were differentially expressed only under ACC conditions. We also report changes in tasi RNA and secondary si RNA expression under both stress conditions. Our results reveal possible roles of snc RNA in the regulatory networks associated with tolerance to low temperatures and provide useful information for a more strategic use of genomic resources in potato breeding.