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Isolation and expression analysis of cadmium‐induced genes from Cd/Mn hyperaccumulator Phytolacca americana in response to high Cd exposure
Author(s) -
Zhao H.,
Wei Y.,
Wang J.,
Chai T.
Publication year - 2019
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12908
Subject(s) - biology , suppression subtractive hybridization , hyperaccumulator , reactive oxygen species , gene , gene expression , microbiology and biotechnology , cadmium , botany , cdna library , biochemistry , ecology , materials science , contamination , soil contamination , metallurgy
Phytolacca americana is recognised as a hyperaccumulator that accumulates cadmium (Cd) and manganese (Mn). Although most studies have provided abundant physiological evidence, little is known about the molecular mechanisms of Cd accumulation in P. americana . In this study, Cd‐induced genes were isolated using suppression subtractive hybridisation ( SSH ) library construction, and gene expression patterns under Cd stress were quantified using real‐time quantitative PCR . The functions of Pa GST , Pa MT and PaFe ‐ SOD were confirmed in transformant yeast. Reactive oxygen species ( ROS ) formation and cell death in root tips were detected, and SOD and POD activities in leaf tissue were also analysed. There were about 447 expressed sequence tags ( EST s) identified and confirmed. GO analysis showed those genes were mainly involved in metabolism, cell stress and defence, transcription and translation, signal transduction, transport, energy and ion transport, which formed the basis for a molecular understanding of P. americana Cd tolerance mechanisms. Cd also stimulated ROS formation and modified the antioxidant systems. Taken together, our results indicate that ROS formation and Cd‐induced gene expression favour P. americana tolerance by activating the defence system and permitting subsequent adaptation to Cd toxicity.