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Large‐scale screening and characterisation of Lemna aequinoctialis and Spirodela polyrhiza strains for starch production
Author(s) -
Ma Y. B.,
Zhu M.,
Yu C. J.,
Wang Y.,
Liu Y.,
Li M. L.,
Sun Y. D.,
Zhao J. S.,
Zhou G. K.
Publication year - 2018
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12679
Subject(s) - starch , biomass (ecology) , biofuel , botany , biology , aquatic plant , food science , agronomy , microbiology and biotechnology , ecology , macrophyte
Duckweed is considered a promising feedstock for bioethanol production due to its high biomass and starch production. Selection of duckweed strains with high starch accumulation is essential for application of duckweeds to bioethanol production. Geographic differentiation had a large influence on genetic diversity of duckweeds. Biomass production, starch content and starch amount in geographically isolated strains of 20 Lemna aequinoctialis and Spirodela polyrhiza were calculated to evaluate their potential for bioethanol production. The influence of different collection time, culture medium and NaCl concentration on starch accumulation of the best strains were analysed. The results showed that biomass production, starch content and starch production of duckweeds demonstrated clonal dependency. The best strain was L. aequinoctialis 6000, with biomass production of 15.38 ± 1.47 g m −2 , starch content of 28.68 ± 1.10% and starch production of 4.39 ± 0.25 g m −2 . Furthermore, starch content of L. aequinoctialis 6000 was highest after 8 h of light, tap water was the best medium for starch induction, and NaCl did not induce starch accumulation. This study suggests duckweed biomass production and starch production demonstrate clonal dependency, indicating that extensive clonal comparisons will be required to identify the most suitable isolates for duckweed selective breeding for bioethanol.