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Overexpression of AtPAD4 in transgenic Brachypodium distachyon enhances resistance to Puccinia brachypodii
Author(s) -
Wang B.,
Wang N.,
Song N.,
Wang W.,
Wang J.,
Wang X.,
Kang Z.
Publication year - 2017
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12616
Subject(s) - brachypodium distachyon , biology , brachypodium , transgene , gene , genetically modified crops , botany , genetics , genome
Brachypodium distachyon (L.) has recently emerged as a model for temperate grasses for investigating the molecular basis of plant–pathogen interactions. Phytoalexin deficient 4 ( PAD 4 ) plays a regulatory role in mediating expression of genes involved in plant defence. In this research, we generated transgenic B. distachyon plants constitutively overexpressing At PAD 4 . Two transgenic B. distachyon lines were verified using PCR and GUS phenotype. Constitutive expression of At PAD 4 in B. distachyon enhanced resistance to Puccinia brachypodii . P . brachypodii generated less urediniospores on transgenic than on wild‐type plants. At PAD 4 overexpression enhanced salicylic acid ( SA ) levels in B. distachyon ‐infected tissues. qRT ‐ PCR showed that expression of pathogenesis‐related 1 ( PR1 ) and other defence‐related genes were up‐regulated in transformed B. distachyon following infection with P. brachypodii . Our results indicate that At PAD 4 overexpression in B. distachyon plants led to SA accumulation and induced PR gene expression that reduced the rate of colonisation by P. brachypodii .