Premium
Long‐distance movement of phosphate starvation‐responsive microRNAs in Arabidopsis
Author(s) -
Huen A. K.,
RodriguezMedina C.,
Ho A. Y. Y.,
Atkins C. A.,
Smith P. M. C.
Publication year - 2017
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12568
Subject(s) - biology , arabidopsis , microrna , starvation response , phosphate , hypocotyl , microbiology and biotechnology , chromosomal translocation , phloem , shoot , mutant , starvation , small rna , botany , genetics , biochemistry , gene , endocrinology
Plant micro RNA s are small RNA s that are important for genetic regulation of processes such as plant development or environmental responses. Specific micro RNA s accumulate in the phloem during phosphate starvation, and may act as long‐distance signalling molecules. We performed quantitative PCR on Arabidopsis hypocotyl micrograft tissues of wild‐type and hen1‐6 mutants to assess the mobility of several phosphate starvation‐responsive micro RNA species. In addition to the previously confirmed mobile species miR399d, the corresponding micro RNA * (miR399d*) was identified for the first time as mobile between shoots and roots. Translocation by phosphate‐responsive micro RNA s miR827 and miR2111a between shoots and roots during phosphate starvation was evident, while their respective micro RNA *s were not mobile. The results suggest that long‐distance mobility of micro RNA species is selective and can occur without the corresponding duplex strand. Movement of miR399d* and root‐localised accumulation of miR2111a* opens the potential for persisting micro RNA *s to be mobile and functional in novel pathways during phosphate starvation responses.