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A transcriptome‐wide study on the micro RNA ‐ and the Argonaute 1‐enriched small RNA ‐mediated regulatory networks involved in plant leaf senescence
Author(s) -
Qin J.,
Ma X.,
Yi Z.,
Tang Z.,
Meng Y.
Publication year - 2016
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12373
Subject(s) - biology , arabidopsis , small rna , rna , argonaute , transcriptome , senescence , gene , transfer rna , genetics , arabidopsis thaliana , microrna , small interfering rna , microbiology and biotechnology , gene expression , mutant
Leaf senescence is an important physiological process during the plant life cycle. However, systemic studies on the impact of micro RNA s (mi RNA s) on the expression of senescence‐associated genes ( SAG s) are lacking. Besides, whether other Argonaute 1 ( AGO 1)‐enriched small RNA s ( sRNA s) play regulatory roles in leaf senescence remains unclear. In this study, a total of 5,123 and 1,399 AGO 1‐enriched sRNA s, excluding mi RNA s, were identified in Arabidopsis thaliana and rice ( Oryza sativa ), respectively. After retrieving SAG s from the Leaf Senescence Database, all of the AGO 1‐enriched sRNA s and the mi RB ase‐registered mi RNA s of these two plants were included for target identification. Supported by degradome signatures, 200 regulatory pairs involving 120 AGO 1‐enriched sRNA s and 40 SAG s, and 266 regulatory pairs involving 64 mi RNA s and 42 SAG s were discovered in Arabidopsis . Moreover, 13 genes predicted to interact with some of the above‐identified target genes at protein level were validated as regulated by 17 AGO 1‐enriched sRNA s and ten mi RNA s in Arabidopsis . In rice, only one SAG was targeted by three AGO 1‐enriched sRNA s, and one SAG was targeted by miR395. However, five AGO 1‐enriched sRNA s were conserved between Arabidopsis and rice. Target genes conserved between the two plants were identified for three of the above five sRNA s, pointing to the conserved roles of these regulatory pairs in leaf senescence or other developmental procedures. Novel targets were discovered for three of the five AGO 1‐enriched sRNA s in rice, indicating species‐specific functions of these sRNA –target pairs. These results could advance our understanding of the sRNA ‐involved molecular processes modulating leaf senescence.