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Isolation and characterisation of c DNA encoding a wheat heavy metal‐associated isoprenylated protein involved in stress responses
Author(s) -
Zhang X.,
Feng H.,
Feng C.,
Xu H.,
Huang X.,
Wang Q.,
Duan X.,
Wang X.,
Wei G.,
Huang L.,
Kang Z.
Publication year - 2015
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12344
Subject(s) - biology , microbiology and biotechnology , abiotic stress , gene , green fluorescent protein , schizosaccharomyces pombe , prenylation , abiotic component , complementary dna , cytosol , guard cell , biochemistry , botany , saccharomyces cerevisiae , enzyme , paleontology
In cells, metallochaperones are important proteins that safely transport metal ions. Heavy metal‐associated isoprenylated plant proteins (HIPPs) are metallochaperones that contain a metal binding domain and a CaaX isoprenylation motif at the carboxy‐terminal end. To investigate the roles of wheat heavy metal‐associated isoprenylated plant protein (Ta HIPP ) genes in plant development and in stress responses, we isolated cDNA encoding the wheat Ta HIPP 1 gene, which contains a heavy metal‐associated domain, nuclear localisation signals and an isoprenylation motif (CaaX motif). Quantitative real‐time PCR analysis indicated that the Ta HIPP 1 gene was differentially expressed under biotic and abiotic stresses. Specifically, Ta HIPP 1 expression was up‐regulated by ABA exposure or wounding. Additionally, Ta HIPP 1 over‐expression in yeast ( Schizosaccharomyces pombe ) significantly increased the cell growth rate under Cu 2+ and high salinity stresses. The nuclear localisation of the protein was confirmed with confocal laser scanning microscopy of epidermal onion cells after particle bombardment with chimeric Ta HIPP 1‐ GFP constructs. In addition, Ta HIPP 1 was shown to enhance the susceptibility of wheat to Pst as determined by virus‐induced gene silencing. These data indicate that Ta HIPP 1 is an important component in defence signalling pathways and may play a crucial role in the defence response of wheat to biotic and certain abiotic stresses.