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Histone deacetylation modification participates in the repression of peanut ( Arachis hypogaea L.) seed storage protein gene Ara h 2.02 during germination
Author(s) -
Yang P.,
Zhang F.,
Luo X.,
Zhou Y.,
Xie J.
Publication year - 2015
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12268
Subject(s) - biology , acetylation , histone deacetylase , chromatin immunoprecipitation , histone , ectopic expression , psychological repression , arachis hypogaea , histone deacetylase 2 , microbiology and biotechnology , chromatin , sap30 , reporter gene , histone h2a , gene expression , gene , genetics , promoter , botany
Genes encoding seed storage proteins ( SSP s) are specifically and highly expressed during seed maturation. In Arabidopsis , chromatin‐based mechanisms involved in the repression of SSP s during germination have been proposed. However, epigenetic regulation involved in repressing SSP s in vegetative tissues of peanut is not well understood. Histone deacetylase ( HDAC ) is a chromatin‐remodelling factor that contributes to transcriptional repression in eukaryotes. To address whether histone deacetylation modification is involved in the repression of SSP genes during germination in peanut, we generated an Ara h 2.02 pro : β ‐glucuronidase ( GUS ) construct by fusing the 1972 bp Ara h 2.02 promoter of peanut (from −1972 to −1) to the GUS reporter gene and transformed it into wild‐type Arabidopsis plants and HDAC mutants. GUS staining revealed that the mutation in HISTONE DEACETYLASE 19 ( HDA 19 ) resulted in the ectopic expression of peanut SSP gene Ara h 2.02 in seedlings. In addition, Chromatin immunoprecipitation (Ch IP ) assays showed that the ectopic expression of Ara h 2.02 was accompanied by histone hyperacetylation during germination. These results suggest that histone deacetylation modification may play a vital role in repressing embryonic properties during the peanut vegetative growth.