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A landscape of hairy and twisted: hunting for new trichome mutants in the S askatoon A rabidopsis T‐ DNA population
Author(s) -
Taheri A.,
Gao P.,
Yu M.,
Cui D.,
Regan S.,
Parkin I.,
Gruber M.
Publication year - 2015
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12230
Subject(s) - trichome , biology , mutant , gene , genetics , phenotype , population , function (biology) , allele , botany , demography , sociology
A total of 88 new A rabidopsis lines with trichome variation were recovered by screening 49,200 single‐seed descent T 3 lines from the SK activation‐tagged population and from a new 20,000‐line T‐ DNA insertion population (called pAG ). Trichome variant lines were classified into 12 distinct phenotype categories. Single or multiple T‐ DNA insertion sites were identified for 89% of these mutant lines. Alleles of the well‐known trichome genes TRY , GL 2 and TTG 1 were recovered with atypical phenotype variation not reported previously. Moreover, atypical gene expression profiles were documented for two additional mutants specifying TRY and GL 2 disruptions. In remaining mutants, ten lines were disrupted in genes coding for proteins not implicated in trichome development, five were disrupted in hypothetical proteins and 11 were disrupted in proteins with unknown function. The collection represents new opportunities for the plant biology community to define trichome development more precisely and to refine the function of individual trichome genes.