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Evaluation of phylogenetic relationships in L emnaceae using nuclear ribosomal data
Author(s) -
Tippery N. P.,
Les D. H.,
Crawford D. J.
Publication year - 2015
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12203
Subject(s) - biology , internal transcribed spacer , phylogenetic tree , ribosomal dna , ribosomal rna , sanger sequencing , dna sequencing , nuclear dna , chloroplast dna , phylogenetics , genetics , dna , gene , mitochondrial dna
Nuclear DNA sequence data are essential for obtaining a complete understanding of plant species relationships, yet these data have been conspicuously absent from phylogenetic analyses of Lemnaceae (duckweeds). Using a modified Sanger sequencing protocol, we obtained DNA sequences of duckweed nuclear ribosomal regions, including 18S and 26S r DNA genes, the external transcribed spacer ( ETS ) and the frequently used internal transcribed spacer ( ITS ). After obtaining sequence data for all Lemnaceae species, we ascertained that prior difficulty in sequencing the ITS regions likely resulted from extremely rigid secondary structures, precipitated by a high proportion of G / C nucleotides. In phylogenetic analyses, nuclear ribosomal data largely supported relationships that had been inferred using chloroplast DNA sequence data.