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Ultraviolet radiation induces stress in etiolated L andoltia punctata , as evidenced by the presence of alanine, a universal stress signal: a 15 N NMR study
Author(s) -
Monselise E. B.I.,
Levkovitz A.,
Kost D.
Publication year - 2015
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12198
Subject(s) - alanine , biology , radical , biochemistry , amino acid
Analysis with 15 N NMR revealed that alanine, a universal cellular stress signal, accumulates in etiolated duckweed plants exposed to 15‐min pulsed UV light, but not in the absence of UV irradiation. The addition of 10 m m vitamin C , a radical scavenger, reduced alanine levels to zero, indicating the involvement of free radicals. Free D ‐alanine was detected in 15 N NMR analysis of the chiral amino acid content, using D ‐tartaric acid as solvent. The accumulation of D ‐alanine under stress conditions presents a new perspective on the biochemical processes taking place in prokaryote and eukaryote cells.