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Regulation of epinasty induced by 2,4‐dichlorophenoxyacetic acid in pea and A rabidopsis plants
Author(s) -
Pazmiño D. M.,
RodríguezSerrano M.,
Sanz M.,
RomeroPuertas M. C.,
Sandalio L. M.
Publication year - 2014
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12128
Subject(s) - abscisic acid , biology , jasmonic acid , fluridone , reactive oxygen species , microbiology and biotechnology , biochemistry , ethylene , arabidopsis , phosphorylation , botany , mutant , salicylic acid , gene , catalysis
The herbicide 2,4‐dichlorophenoxyacetic acid (2,4‐ D ) causes uncontrolled cell division and malformed growth in plants, giving rise to leaf epinasty and stem curvature. In this study, mechanisms involved in the regulation of leaf epinasty induced by 2,4‐ D were studied using different chemicals involved in reactive oxygen species ( ROS ) accumulation (diphenyleniodonium, butylated hydroxyanisole, EDTA , allopurinol), calcium channels ( L a C l 3 ), protein phosphorylation (cantharidin, wortmannin) and ethylene emission/perception (aminoethoxyvinyl glycine, A g NO 3 ). The effect of these compounds on the epinasty induced by 2,4‐ D was analysed in shoots and leaf strips from pea plants. For further insight into the effect of 2,4‐ D , studies were also made in A rabidopsis mutants deficient in ROS production ( rboh D , rboh F , xdh ), ethylene (ein 3‐1, ctr 1‐1, etr 1‐1 ), abscisic acid ( aba 3.1 ), and jasmonic acid ( coi 1.1, jar 1.1, opr 3 ) pathways. The results suggest that ROS production, mainly ·OH , is essential in the development of epinasty triggered by 2,4‐ D . Epinasty was also found to be regulated by C a 2+ , protein phosphorylation and ethylene, although all these factors act downstream of ROS production. The use of A rabidopsis mutants appears to indicate that abscisic and jasmonic acid are not involved in regulating epinasty, although they could be involved in other symptoms induced by 2,4‐ D .