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Effects of 24‐epibrassinolide on plant growth, osmotic regulation and ion homeostasis of salt‐stressed canola
Author(s) -
Liu J.,
Gao H.,
Wang X.,
Zheng Q.,
Wang C.,
Wang X.,
Wang Q.
Publication year - 2014
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12052
Subject(s) - canola , biology , proline , shoot , osmotic pressure , salt (chemistry) , osmoregulation , horticulture , inorganic ions , salinity , botany , amino acid , ion , chemistry , biochemistry , ecology , organic chemistry
This study evaluated effects of foliar spraying 24‐epibrassinoide (24‐ EBL ) on the growth of salt‐stressed canola. Seedlings at the four‐leaf stage were treated with 150 m m N a C l and different concentrations of 24‐ EBL (10 −6 , 10 −8 , 10 −10 , 10 −12 m ) for 15 days. A concentration of 10 −10 m 24‐ EBL was chosen as optimal and used in a subsequent experiment on plant biomass and leaf water potential parameters. The results showed that 24‐ EBL mainly promoted shoot growth of salt‐stressed plants and also ameliorated leaf water status. Foliar spraying of salt‐stressed canola with 24‐ EBL increased osmotic adjustment ability in all organs, especially in younger leaves and roots. This was mainly due to an increase of free amino acid content in upper leaves, soluble sugars in middle leaves, organic acids and proline in lower leaves, all of these compounds in roots, as well as essential inorganic ions. N a + and C l − sharply increased in different organs under salt stress, and 24‐ EBL reduced their accumulation. 24‐ EBL improved the uptake of K + , C a 2+ , M g 2+ and NO 3 − in roots, which were mainly transported to upper leaves, while NO 3 − was mainly transported to middle leaves. Thus, 24‐ EBL improvements in ion homeostasis of K + / N a + , C a 2+ / N a + , M g 2+ / N a + and NO 3 − / C l − , especially in younger leaves and roots, could be explained. As most important parts, younger leaves and roots were the main organs protected by 24‐ EBL via improvement in osmotic adjustment ability and ion homeostasis. Further, physiological status of growth of salt‐stressed canola was ameliorated after 24‐ EBL treatment.