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Quantitative trait loci and candidate genes associated with starch pasting viscosity characteristics in cassava ( M anihot esculenta C rantz)
Author(s) -
Thanyasiriwat T.,
Sraphet S.,
Whankaew S.,
Boonseng O.,
Bao J.,
Lightfoot D. A.,
Tangphatsornruang S.,
Triwitayakorn K.
Publication year - 2014
Publication title -
plant biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.871
H-Index - 87
eISSN - 1438-8677
pISSN - 1435-8603
DOI - 10.1111/plb.12022
Subject(s) - quantitative trait locus , biology , candidate gene , population , starch , genetic linkage , trait , gene , cultivar , genetics , botany , food science , demography , sociology , computer science , programming language
Starch pasting viscosity is an important quality trait in cassava ( M anihot esculenta C rantz) cultivars. The aim here was to identify loci and candidate genes associated with the starch pasting viscosity. Quantitative trait loci ( QTL ) mapping for seven pasting viscosity parameters was carried out using 100 lines of an F 1 mapping population from a cross between two cassava cultivars H uay B ong 60 and H anatee. Starch samples were obtained from roots of cassava grown in 2008 and 2009 at R ayong, and in 2009 at L op B uri province, Thailand. The traits showed continuous distribution among the F 1 progeny with transgressive variation. Fifteen QTL were identified from mean trait data, with Logarithm of Odds ( LOD ) values from 2.77–13.01 and phenotype variations explained ( PVE ) from10.0–48.4%. In addition, 48 QTL were identified in separate environments. The LOD values ranged from 2.55–8.68 and explained 6.6–43.7% of phenotype variation. The loci were located on 19 linkage groups. The most important QTL for pasting temperature ( PT ) ( q PT .1 LG 1 ) from mean trait values showed largest effect with highest LOD value (13.01) and PVE (48.4%). The QTL co‐localised with PT and pasting time ( PT i) loci that were identified in separate environments. Candidate genes were identified within the QTL peak regions. However, the major genes of interest, encoding the family of glycosyl or glucosyl transferases and hydrolases, were located at the periphery of QTL peaks. The loci identified could be effectively applied in breeding programmes to improve cassava starch quality. Alleles of candidate genes should be further studied in order to better understand their effects on starch quality traits.