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Experimental pathology by intravital microscopy and genetically encoded fluorescent biosensors
Author(s) -
Matsuda Michiyuki,
Terai Kenta
Publication year - 2020
Publication title -
pathology international
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.73
H-Index - 74
eISSN - 1440-1827
pISSN - 1320-5463
DOI - 10.1111/pin.12925
Subject(s) - förster resonance energy transfer , microscope , two photon excitation microscopy , microscopy , intravital microscopy , biosensor , fluorescence microscope , genetically engineered , multiphoton fluorescence microscope , nanotechnology , live cell imaging , biology , fluorescence , pathology , medicine , materials science , cell , optics , physics , biochemistry , gene , microbiology and biotechnology , in vivo
The invention of two‐photon excitation microscopes widens the potential application of intravital microscopy (IVM) to the broad field of experimental pathology. Moreover, the recent development of fluorescent protein‐based, genetically encoded biosensors provides an ideal tool to visualize the cell function in live animals. We start from a brief review of IVM with two‐photon excitation microscopes and genetically encoded biosensors based on the principle of Förster resonance energy transfer (FRET). Then, we describe how IVM using biosensors has revealed the pathogenesis of several disease models.